Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A antibody specific to AChE has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated AChE and incubated. A competitive inhibition reaction is launched between AChE (Standards or samples) and Biotin-conjugated AChE with the pre-coated antibody specific for AChE. The more amount of AChE in samples, the less antibody bound by Biotin-conjugated AChE. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of AChE in the sample. The color development is stopped and the intensity of the color is measured